Error Frequency in 5-S RNA from Cells Grown in 5-Bromodeoxyuridine
نویسندگان
چکیده
منابع مشابه
Relationship of antigenicity of melanoma cells grown in 5-bromodeoxyuridine to reduced tumorigenicity.
B16 mouse melanoma cells (clone BS59) grown in 1 to 3 ¿ig of 5-bromodeoxyuridine (BUdR) per ml form few or no tumors in C57BL/6J adult mice when injected subcutaneously in doses at which untreated cells always form tumors. Injections of BUdR-grown cells protect adult mice against melanoma. Melanoma cells grown in 1/ig of BUdR per ml for almost 1 year (clone C3471) retain a plating efficiency s...
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• >v r ABSTRACT ' v Chemical modification and limited enzymatic hydrolysis on isolated E. coli 5S RNA have provided informations on the * secondary-and tertiary structure compatible with pseudoknotted structures for the A-and B-conformers of the molecule. * Changes in the accessibility and reactivity of nucleotides * in loop C and at the stem of helix IV in two different 5S RNA *" conformers ar...
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The thymidine analog 5-bromodeoxyuridine (BrUdR) incorporated into DNA has been successfully used as a selective agent (1, 2) or mutagen (3) on various animal cells. Some of its effects are understandable in terms of base substitution in the genetic material; others, however, such as the reversible suppression of cytodifferentiation (4-7), remain unexplained. We have examined the effects of pro...
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Low concentrations (1-3 microg/ml) of 5-bromodeoxyuridine (BrdU) reversibly suppress pigmentation in a highly pigmented clone (B(5)59) of cultured B16 mouse melanoma cells. We have found that unpigmented cells (clone C(3)471), derived by long-term culture of B(5)59 cells in 1 microg of BrdU/ml, were completely amelanotic with no biochemically or cytochemically detectable tyrosinase activity or ...
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Epstein-Barr virus was observed by electron microscopy in human lympho blastoid cells derived from a rhabdomyosarcoma. Replication of virus particles was stimulated approximately 1 0 fold after treatment of the cells with either 5'-iododeoxyuridine or 5'-bromodeoxyuridine. The evidence supports the con cept that the entire Epstein-Barr virus genome persists in some cells of this type. The dat...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1974
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1974.tb03768.x